Composite

Part:BBa_K3286116:Design

Designed by: Robert Hooftman   Group: iGEM19_Wageningen_UR   (2019-10-19)


Expression cassette for Chitin binding domain Chitinase A1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

See individual parts


Source

See individual parts

References

  • V. K. Mutalik et al., “Precise and reliable gene expression via standard transcription and translation initiation elements,” Nat. Methods, vol. 10, no. 4, pp. 354–360, Apr. 2013.
  • M. Hashimoto et al., “Expression and characterization of the chitin-binding domain of chitinase A1 from Bacillus circulans WL-12.,” J. Bacteriol., vol. 182, no. 11, pp. 3045–54, Jun. 2000.
  • Nieuwkoop, T., Claassens, N. J., & van der Oost, J. (2019). Improved protein production and codon optimization analyses in Escherichia coli by bicistronic design. Microbial biotechnology, 12(1), 173-179.